Characterization of maize root microbiome in two different soils by minimizing plant DNA contamination in metabarcoding analysis

Abstract A micropore-filtration method was used to reduce the proportion of plant DNA in microbial samples isolated from roots prior sequencing. We tested impact this pre-sequencing filtration methodology and it characterize root microbiome maize grown on two soils with different fertility levels. The micropore reduced contamination unveiled potential N-poor soil for N fixation phosphate uptake by phosphate-poor soil. Our findings allude capability plants initiate plant-microbe interactions under sub-optimal fertility.